Existing studies predominantly focus on cryptographic vulnerabilities affecting confidentiality or integrity, with limited attention to those impacting availability. To fill this gap, we conduct a comprehensive study targeting implementations vulnerable to DoS (Denial-of-Service) attacks within cryptographic libraries. Notably, we observed that these vulnerable implementations are frequently associated, directly or indirectly, with X.509 certificates. Consequently, we facilitate the launch of DoS attacks by using crafted X.509 certificates as attack vectors, which we termed X.509DoS in this work.